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gene pulser xcell  (Bio-Rad)


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    Structured Review

    Bio-Rad gene pulser xcell
    Gene Pulser Xcell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 5903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gene+pulser/Gene+Pulser+Xcell+Total+System/pmc13123328-132-53-56
    Average 97 stars, based on 5903 article reviews
    gene pulser xcell - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Boosting Recombinant Bovine Chymosin in Komagataella phaffii via Fusion Protein and Constitutive Promoter Expression.
    Article Snippet: The expression vectors pPIC9-pcm14, pPIC9-clpcm14, and pGAPZα-clpcm14 were linearized with restriction enzymes Bgl II and Avr II (Thermo Fisher Scientific, Waltham, MA, USA). .. The linearized DNA was then transformed into K. phaffii GS115 via electroporation using a Gene Pulser (Bio-Rad Laboratories, Hercules, CA, USA) with 2 mm gap cuvettes under the following conditions: 2000 V, 200 Ω, 50 μF. ..

    Electroporation:

    Article Title: Boosting Recombinant Bovine Chymosin in Komagataella phaffii via Fusion Protein and Constitutive Promoter Expression.
    Article Snippet: The expression vectors pPIC9-pcm14, pPIC9-clpcm14, and pGAPZα-clpcm14 were linearized with restriction enzymes Bgl II and Avr II (Thermo Fisher Scientific, Waltham, MA, USA). .. The linearized DNA was then transformed into K. phaffii GS115 via electroporation using a Gene Pulser (Bio-Rad Laboratories, Hercules, CA, USA) with 2 mm gap cuvettes under the following conditions: 2000 V, 200 Ω, 50 μF. ..

    Article Title: Functional Analysis of Naturally Integrated Rol Genes in Sweet Potato via CRISPR/Cas9 Genome Editing
    Article Snippet: .. The final constructs, pPZP-RCS2-NPTII/pcoCas9/gRNA1 and pPZP-RCS2-NPTII/pcoCas9/gRNA2, were introduced into Agrobacterium tumefaciens strain EHA105/pTiBo542 by electroporation using the Gene Pulser (Bio-Rad Laboratories, Hercules, CA, USA), following the manufacturer’s protocol. ..

    Article Title: Techno-economic analysis of industrial-scale fermentation for formate dehydrogenase (FDH) production
    Article Snippet: .. Electroporation was performed using a Gene Pulser (Bio-Rad) with settings of 2.50 kV, 400 Ω, and 25 μF for a 2-mm gap cuvette. ..

    Article Title: A genome-wide CRISPR screen identifies GRA38 as a key regulator of lipid homeostasis during Toxoplasma gondii adaptation to lipid-rich conditions
    Article Snippet: .. Electroporation was conducted using the Gene Pulser (Bio-Rad) at settings of 25 mFD, 1250 V, and ∞ Ω, with a single pulse. ..

    Article Title: Porphyromonas gingivalis induces intestinal inflammation through gingipain-dependent gut microbiome dysbiosis.
    Article Snippet: .. The pelleted cells were washed three times with pre- cooled electroporation buffer (10% glycerol, 1 mM MgCl2), resuspended in 100 μL electroporation buffer, and mixed with 2 μg DNA fragment, followed by electroporation at 2500 V using Gene Pulser (Bio-Rad). ..

    Plasmid Preparation:

    Article Title: The apoplastic protein OsCPIP8 confers dual protection by inhibiting pathogenic protease and activating rice immunity.
    Article Snippet: .. Approximately 490 2 μg of plasmid DNA was mixed with 100 μL of competent RS105 cells and 491 electroporated using a Gene Pulser (Bio-Rad). ..

    Construct:

    Article Title: Functional Analysis of Naturally Integrated Rol Genes in Sweet Potato via CRISPR/Cas9 Genome Editing
    Article Snippet: .. The final constructs, pPZP-RCS2-NPTII/pcoCas9/gRNA1 and pPZP-RCS2-NPTII/pcoCas9/gRNA2, were introduced into Agrobacterium tumefaciens strain EHA105/pTiBo542 by electroporation using the Gene Pulser (Bio-Rad Laboratories, Hercules, CA, USA), following the manufacturer’s protocol. ..

    Knock-Out:

    Article Title: Queuosine promotes wecB -dependent phage resistance and biofilm formation in marine bacterium Shewanella glacialimarina
    Article Snippet: .. Knockout vectors (2 μg) were electroporated to 50 μL S. glacialimarina competent cells in pre-cooled 0.1 cm cuvettes using Gene Pulser (Biorad) using 12.5 kV/cm, 800 Ω. .. Cells were recovered in 1 mL of 25% rMB for 2 h in table-top thermomixer (Eppendorf) at +15 °C and 900 rpm.



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    Schematic illustration of the preparation of M2-exo@HI and its mediated therapeutic mechanisms and signaling pathways. (a) RAW264.7 macrophages were polarized to M2 phenotype using DEX, followed by M2-exo isolation from cell supernatant via differential centrifugation. M2-exo@HI was prepared through encapsulating HI into M2-exo by <t>electroporation.</t> (b) M2-exo@HI crossed the BBB and localized to microglia in the hemorrhagic brain, delivering the HI plasmid into the nucleus. This prompted expression and secretion of Hp and IL-10 by microglia. The released Hp inhibited Hb toxicity by binding to Hb. IL-10 shifted microglial polarization from the pro-inflammatory M1 phenotype toward the reparative M2 phenotype, removing hematoma by engulfing erythrocyte and Hb-Hp complex, and decreasing pro-inflammatory cytokine levels—collectively enhancing neuroprotection and BBB repair. These beneficial outcomes were linked to the inhibition of the IL-17 and NF-κB signaling pathways and activation of the PI3K-Akt and ferroptosis pathways.
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    Bio-Rad bio rad gene pulser xcell device
    Schematic illustration of the preparation of M2-exo@HI and its mediated therapeutic mechanisms and signaling pathways. (a) RAW264.7 macrophages were polarized to M2 phenotype using DEX, followed by M2-exo isolation from cell supernatant via differential centrifugation. M2-exo@HI was prepared through encapsulating HI into M2-exo by <t>electroporation.</t> (b) M2-exo@HI crossed the BBB and localized to microglia in the hemorrhagic brain, delivering the HI plasmid into the nucleus. This prompted expression and secretion of Hp and IL-10 by microglia. The released Hp inhibited Hb toxicity by binding to Hb. IL-10 shifted microglial polarization from the pro-inflammatory M1 phenotype toward the reparative M2 phenotype, removing hematoma by engulfing erythrocyte and Hb-Hp complex, and decreasing pro-inflammatory cytokine levels—collectively enhancing neuroprotection and BBB repair. These beneficial outcomes were linked to the inhibition of the IL-17 and NF-κB signaling pathways and activation of the PI3K-Akt and ferroptosis pathways.
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    Image Search Results


    Schematic illustration of the preparation of M2-exo@HI and its mediated therapeutic mechanisms and signaling pathways. (a) RAW264.7 macrophages were polarized to M2 phenotype using DEX, followed by M2-exo isolation from cell supernatant via differential centrifugation. M2-exo@HI was prepared through encapsulating HI into M2-exo by electroporation. (b) M2-exo@HI crossed the BBB and localized to microglia in the hemorrhagic brain, delivering the HI plasmid into the nucleus. This prompted expression and secretion of Hp and IL-10 by microglia. The released Hp inhibited Hb toxicity by binding to Hb. IL-10 shifted microglial polarization from the pro-inflammatory M1 phenotype toward the reparative M2 phenotype, removing hematoma by engulfing erythrocyte and Hb-Hp complex, and decreasing pro-inflammatory cytokine levels—collectively enhancing neuroprotection and BBB repair. These beneficial outcomes were linked to the inhibition of the IL-17 and NF-κB signaling pathways and activation of the PI3K-Akt and ferroptosis pathways.

    Journal: Bioactive Materials

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage

    doi: 10.1016/j.bioactmat.2026.01.047

    Figure Lengend Snippet: Schematic illustration of the preparation of M2-exo@HI and its mediated therapeutic mechanisms and signaling pathways. (a) RAW264.7 macrophages were polarized to M2 phenotype using DEX, followed by M2-exo isolation from cell supernatant via differential centrifugation. M2-exo@HI was prepared through encapsulating HI into M2-exo by electroporation. (b) M2-exo@HI crossed the BBB and localized to microglia in the hemorrhagic brain, delivering the HI plasmid into the nucleus. This prompted expression and secretion of Hp and IL-10 by microglia. The released Hp inhibited Hb toxicity by binding to Hb. IL-10 shifted microglial polarization from the pro-inflammatory M1 phenotype toward the reparative M2 phenotype, removing hematoma by engulfing erythrocyte and Hb-Hp complex, and decreasing pro-inflammatory cytokine levels—collectively enhancing neuroprotection and BBB repair. These beneficial outcomes were linked to the inhibition of the IL-17 and NF-κB signaling pathways and activation of the PI3K-Akt and ferroptosis pathways.

    Article Snippet: M2-exo (100 μL, 1 mg/mL), HI plasmid (circular pBudCE4.1, 100 μg, TsingkeBiotechnology., Ltd.), and 100 μL PBS were added to the electroporation cuvettes (Bio-Rad 165-2088).

    Techniques: Protein-Protein interactions, Isolation, Centrifugation, Electroporation, Plasmid Preparation, Expressing, Binding Assay, Inhibition, Activation Assay