gene pulser xcell (Bio-Rad)
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Gene Pulser Xcell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 5903 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pulser/Gene+Pulser+Xcell+Total+System/pmc13123328-132-53-56
Average 97 stars, based on 5903 article reviews
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Transformation Assay:Article Title: Boosting Recombinant Bovine Chymosin in Komagataella phaffii via Fusion Protein and Constitutive Promoter Expression. Article Snippet: The expression vectors pPIC9-pcm14, pPIC9-clpcm14, and pGAPZα-clpcm14 were linearized with restriction enzymes Bgl II and Avr II (Thermo Fisher Scientific, Waltham, MA, USA). .. The linearized DNA was then transformed into K. phaffii GS115 via electroporation using a Electroporation:Article Title: Boosting Recombinant Bovine Chymosin in Komagataella phaffii via Fusion Protein and Constitutive Promoter Expression. Article Snippet: The expression vectors pPIC9-pcm14, pPIC9-clpcm14, and pGAPZα-clpcm14 were linearized with restriction enzymes Bgl II and Avr II (Thermo Fisher Scientific, Waltham, MA, USA). .. The linearized DNA was then transformed into K. phaffii GS115 via electroporation using a Article Title: Functional Analysis of Naturally Integrated Rol Genes in Sweet Potato via CRISPR/Cas9 Genome Editing Article Snippet: .. The final constructs, pPZP-RCS2-NPTII/pcoCas9/gRNA1 and pPZP-RCS2-NPTII/pcoCas9/gRNA2, were introduced into Agrobacterium tumefaciens strain EHA105/pTiBo542 by electroporation using the Article Title: Techno-economic analysis of industrial-scale fermentation for formate dehydrogenase (FDH) production Article Snippet: .. Electroporation was performed using a Article Title: A genome-wide CRISPR screen identifies GRA38 as a key regulator of lipid homeostasis during Toxoplasma gondii adaptation to lipid-rich conditions Article Snippet: .. Electroporation was conducted using the Article Title: Porphyromonas gingivalis induces intestinal inflammation through gingipain-dependent gut microbiome dysbiosis. Article Snippet: .. The pelleted cells were washed three times with pre- cooled electroporation buffer (10% glycerol, 1 mM MgCl2), resuspended in 100 μL electroporation buffer, and mixed with 2 μg DNA fragment, followed by electroporation at 2500 V using Plasmid Preparation:Article Title: The apoplastic protein OsCPIP8 confers dual protection by inhibiting pathogenic protease and activating rice immunity. Article Snippet: .. Approximately 490 2 μg of plasmid DNA was mixed with 100 μL of competent RS105 cells and 491 electroporated using a Construct:Article Title: Functional Analysis of Naturally Integrated Rol Genes in Sweet Potato via CRISPR/Cas9 Genome Editing Article Snippet: .. The final constructs, pPZP-RCS2-NPTII/pcoCas9/gRNA1 and pPZP-RCS2-NPTII/pcoCas9/gRNA2, were introduced into Agrobacterium tumefaciens strain EHA105/pTiBo542 by electroporation using the Knock-Out:Article Title: Queuosine promotes wecB -dependent phage resistance and biofilm formation in marine bacterium Shewanella glacialimarina Article Snippet: .. Knockout vectors (2 μg) were electroporated to 50 μL S. glacialimarina competent cells in pre-cooled 0.1 cm cuvettes using |
